![]() Intrinsically photosensitive retinal ganglion cells (ipRGC) in the Unlike ChR2, the photopigment melanopsin is expressed by Of note, ChR2 has been demonstrated toīe able to restore the function of degenerative retinal diseases. Which was first described in green algae, is a light-gated ionĬhannel which evokes ultrafast membrane depolarization and mediatesĪn action potential ( 7, 8). Melanopsin have recently emerged as a hotspot of RP research. Sensitivity-enhancing proteins such as channel rhodopsins (ChR) and ( 2, 3), stem-cell transplantation ( 4), gene therapy ( 5) and light sensitivity-modulating Have advanced in recent years, which include drug treatments Retinitis pigmentosa (RP) is an pathologicalĬondition leading to progressive visual decline resulting fromĬontinual loss of photoreceptor cells and outer nuclear layers ofĬures for this degenerative retinal disease are available.Įxperimental approaches for the development of treatments of RP In conclusion, the present study showed that during retinal degeneration, the expression of melanopsin was significantly decreased, while vector-mediated overexpression of melanopsin delayed the loss of visual function in rats. In a flash electroretinography experiment, the b‑wave and response to light flash stimuli at three and five weeks following injection with AV‑OPN4‑GFP were higher compared to those in eyes injected with AV‑GFP (P<0.05). Animals injected into both eyes were subjected to a behavioral open-field test, revealing that melanopsin overexpression reduced the loss of light sensitivity of the rats. Immunohistochemical and western blot analyses were used to detect the distribution and protein expression of melanopsin in the retina, revealing that melanopsin was gradually reduced with increasing age of the rats, which was due to loss of dendritic axons of intrinsically photosensitive retinal ganglion cells. Royal College of Surgeons rats, which were used as an animal model of inherited retinal degeneration, were subjected to sub-retinal injection with melanopsin overexpression vector (AV‑OPN4‑GFP). The aim of the present study was to explore whether melanopsin was able to restore retinal function and inhibit its degeneration by acting in a similar manner to channel rhodopsins. Retinitis pigmentosa (RP) is a pathological condition leading to progressive visual decline resulting from continual loss of photoreceptor cells and outer nuclear layers of the retina.
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